Abstract : The present study has been designed to investigate wheather Nano-piprine and Vit. C have improving role in immunity system. The study has been conducted on forty-eight mature male Wistar rats (aged 90 days and weighted 150±10 g) have been randomly assigned to 6 equal groups (8 per group) and dosed orally for 30 consecutive day. The first group (C) was given distilled water as a control group. The second group (T1) was given nano-piperine at a dose of 15 mg/kg of B.W. The third group (T2) was given vitamin C at a dose of 25 mg/kg of B.W. The fourth group (T3) was given dexamethasone at a dose of 2mg/kg of B.W. The fifth group(T4) was given both nano-piperine and dexamethasone at the same time. The sixth group (T5) was given both vitamin C and dexamethasone at the same time. After the end of experiment the animals were dissected and blood samples were collected analyzed WBCs and WBCs differentiation. Blood serum samples were separated for assessment of IL-6, IgG, IgA and IgM concentrations. The results illustrated a significant increase (p<0.05) in WBCs count in T1 and T2 groups, while decreased in T3 as compared with control group. Also there is increased in WBCs in T4 and T5 groups as compared with T3 group. Also the results revealed there was a significant rise (p<0.05) in percentage of lymphocyte and monocyte in T1 and T2 groups while decreased in T3 group as compared with control group. And increased this percentage in T4 and T5 groups as compared with T3 group. The results indicated there was a significant decrease (p<0.05) in percentage of neutrophils in T1,T2 ,T4 and T5,while increased in T3 group.The results registered a significant decrease (p<0.05) in concentration of serum IL-6 in T1,T2 and T3 groups as compared with control group, also decrease in T4 and T5 groups as compared with T3 group. Also the results of IgG, IgA and IgM showed a significant decrease (p<0.05) in T1,T2,and T3 as compared with control group and appeared a significant decrease in T4 and T5 groups as compared with T3 group.