Abstract : Background: Chronic kidney disease (CKD) affects about 5-10% of the global population. As there is decline in renal function, alterations can be observed in acid base balance, fluid and electrolyte homeostasis and decrease in clearance of metabolic wastes like urea, creatinine and uric acid; all of which results in unwanted health complications. Aim: To estimate serum urea, creatinine, uric acid and minerals (calcium, phosphorus, sodium, potassium and chloride) in patients with CKD and to compare that with the healthy controls. Methods: This study was conducted with inclusion of 50 CKD patients and 50 age and sex matched healthy control group. Biochemical investigations such as serum urea, creatinine, uric acid, calcium, phosphorous, chloride, sodium and potassium were carried out. The findings were recorded and results were analyzed using SPSS version 20. Results: Hypertension and diabetes mellitus were the common etiologies of CKD while general weakness, pallor, pedal edema and anorexia were the commonest clinical signs presented. Serum urea, creatinine, uric acid and phosphorous were significantly increased while the levels of calcium, sodium, chloride and potassium were not altered significantly. The levels of significantly altered biochemical parameters showed progressive and significant increase as the renal impairment progressed. Conclusion: CKD is associated with altered blood levels of several biochemical parameters. Hence routine analysis of these parameters aids in the management of CKD patients.