Abstract : Background: The incidence of anterior cruciate ligament (ACL) damage, the necessity of anatomy in surgical repair, and the propensity for the torn ACL to predispose to osteoarthritis all demand a thorough study of its architecture. Aim: To study the morphology and morphometry of Anterior cruciate ligament Methods & Materials: The present study was used 150 anterior cruciate ligaments from 75 human embalmed cadavers of lower limbs obtained from the anatomy departments of Dr. D. Y Patil Medical College in Pune and DVVPF's Medical College and Hospital in Ahmednagar. After remove the skin, fascia and muscles were removed by making a longitudinal incision on each side of the knee joint capsule, identified the Anterior Cruciate ligament Measurements were taken using a digital Vernier Caliper and Statistical analysis was done by descriptive statistics as mean, SD, percentage. Results: Morphological variations of ACL bundles were noted macroscopically. Mean length width and thickness of ACL on the right side was noted as 31.54±2.46, 12.59±2.95, 4.95±1.02 and on the left side it was observed as 30.12 ± 4.4, 12.33±1.18,4.72±0.82mm respectively. Conclusion: The morphological and the morphometric data provided in the present study may be utilized by surgeons to use more anatomically aligned ACL transplants.