Abstract : The present study has been designed to investigate wheather punica granatum has its own antioxidant activity and/or it act through induction of the hypothyroidism. The study has been conducted on adult male rats at the department of physiology, College of Veterinary Medicine, Al-Qadisiya University during the period extended from June, 2022 to December, 2022. Fourty mature female Wistar rats (aged 90 days and weighted 150±10 g) were used in the five experimental periods of the present study. Have been randomly divided randomly into four equal groups, Control group: will give 1ml of distal water daily for 30 days. The first treated group (T1): will give propylthiouracil(PTU) (Singh etal., 2020) daily at dose 8 mg/kg of body weight orally for 30 days. The second treated group (T2): will give polyphenols of punica granatum peel extract (Parmar&Kar,2008) orally at dose 200 mg/kg daily for 30 days. The third treated group (T3): will give (PTU 8mg/kg) and polyphenols of Punica granatum peel extract (200mg/kg) orally for 30 days. The end of each treated and control period, males were anaesthesized (by injection of 0.3ml ketamine + 0.1 ml of xylazine/ kg b.w. ip), dissected and blood samples were obtained from abdominal vein in non-heparinized tubes. Blood serum samples were separated for assessment of GSH, CAT, MDA, cholesterol, Triglyceride, HDL and LDL concentrations. The results of the current study showed that there is an effect of PTU and pomegranate extract on Oxidative-Antioxidants and lipid profile.